IVIS Imaging Service Center
The IVIS Imaging Service Center provides access to fluorescence and luminescence imaging technology through supervised use of the IVIS Lumina III. The Lumina III can be used for imaging of living prokaryotes (e.g., bacterial plates, films), invertebrates (e.g., nematodes, insects), fungi, plants and vertebrates (e.g., fish, mammals, birds) and ex vivo tissue specimens. Some examples from the published literature are provided below.
Have a question or want to schedule a training to use the Lumina III?
If you are interested in using the IVIS Imaging Service Center, you will need to complete the online training and schedule in-person training. Once training is completed, we can schedule your approved experimental protocol that is consistent with IVIS Lumina III capabilities and image analysis. To inquire about training and using the IVIS Lumina III, please contact Shirley Luckhart, Center for Health in the Human Ecosystem, and Kiah Stewart, U of I Lab Animal Research Facility.
Technology Access Grants (TAGs) available
Applications are now open for CHHE IVIS Imaging Service Center Technology Access Grants (TAG). CHHE will fund up to five (5) TAGs for U of I investigators interested in using this facility for experimental research with fluorescence and bioluminescence imaging technology, through supervised use of the IVIS Lumina III. TAG support for up to $1,200 per project will be awarded for hours of training provided by the service center, for use of the IVIS Lumina III and for data analysis completed on service center computers. U of I investigators, postdoctoral fellows, graduate students and other research group members are eligible to apply.
Applications are now open. Applications will be reviewed starting April 15, 2019, and continue until five TAGs are awarded. We anticipate the projects will be implemented during the period from May 15, 2019 through July 31, 2020.
IVIS Imaging Service Center — Your Research in Living Color
Presentation by Shirley Luckhart, Ph.D., CHHE Co-Director, in the IRIC Lunch and Learn Series, March 7, 2019. Download presentation Your Research in Living Color (pptx)
- In vitro Bioluminescence videos | |
- In vitro Fluorescence videos | |
- Advanced imaging of fluorescent and bioluminescent probes (pptx)
- Software manual (pdf)
How can I use the IVIS?
- Bioluminescence imaging of c-fos gene expression accompanying filial imprinting in the newly hatched chick brain (pdf)
- Filial responses as predisposed and learned preferences: Early attachment in chicks and babies (pdf)
- Critical role of the neural pathway from the intermediate medial mesopallium to the intermediate hyperpallium apicale in filial imprinting of domestic chicks (Gallus gallus domesticus) (pdf)
- An ultrasensitive NanoLuc‑based luminescence system for monitoring Plasmodium berghei throughout its life cycle (pdf)
- Bioluminescent imaging of Trypanosoma cruzi infection in Rhodnius prolixus (pdf)
- CpxRA contributes to Xenorhabdus nematophila virulence through regulation of lrhA and modulation of insect immunity (pdf)
- Molecular pathogenesis of Listeria monocytogenes in the alternative model host Galleria mellonella (pdf)
- Monitoring of the parasite load in the digestive tract of Rhodnius prolixus by combined qPCR analysis and imaging techniques provides new insights into the trypanosome life cycle (pdf)
- Paratransgenesis: An approach to improve colony health and molecular insight in honey bees (Apis mellifera)? (pdf)
- Visualization and live imaging analysis of a mosquito saliva protein in host animal skin using a transgenic mosquito with a secreted luciferase reporter system (pdf)
- Long-term luciferase expression monitored by bioluminescence imaging after adeno-associated virus-mediated fetal gene delivery in Rhesus monkeys (Macaca mulatta) (pdf)
- Real-time bioluminescence imaging of macroencapsulated fibroblasts reveals allograft protection in Rhesus monkeys (Macaca mulatta) (pdf)
- Bioluminescence imaging of Clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants (pdf)
- Combined treatments reduce chilling injury and maintain fruit quality in avocado fruit during cold quarantine (pdf)
- Leveraging management strategies for seedborne plant diseases to reduce Salmonella enterica Serovar Typhimurium incidence on tomato seed and seedlings (pdf)
- Using spontaneous photon emission to image lipid oxidation patterns in plant tissues (pdf)
- Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging (pdf)
- Increased anthocyanin and flavonoids in mango fruit peel are associated with cold and pathogen resistance (pdf)